Publications

2024

High-dimensional in situ proteomics imaging to assess γδ T cells in spatial biology

Herold N., Bruhns M., Babaei S., Spreuer J., Castagna A., Yurttas C., Scheuermann S., Seitz C., Ruf B., Königsrainer A., Jurmeister P., Löffler M.W., Claassen M., Wistuba-Hamprecht K.
J Leukoc Biol. 2024 Jan 29

Reprogramming of human γδ T cells by expression of an anti-CD19 TCR fusion construct (εTRuC) to enhance tumor killing

Juraske C., Krissmer S.M., Teuber E.S., Parigiani M.A., Strietz J., Wesch D., Kabelitz D., Minguet S., Schamel W.W.
J Leukoc Biol. 2024 Jan 19;115(2):293-305

Vdelta1 T cells are more resistant than Vdelta2 T cells to the immunosuppressive properties of galectin-3

Schadeck J., Oberg H.H., Peipp M., Hedemann N., Schamel W.W., Bauerschlag D., Wesch D.
Front Immunol. 2024 Jan 8:14:1286097
2023

Dual targeting of cancer metabolome and stress antigens affects transcriptomic heterogeneity and efficacy of engineered T cells

Hernández-López P., van Diest E., Brazda P., Heijhuurs S., Meringa A., Hoorens van Heyningen L.., Riillo C., Schwenzel C., Zintchenko M., Johanna I., J T Nicolasen M., Cleven A., Kluiver T.A., Millen R., Zheng J., Karaiskaki F., Straetemans T., Clevers H., de Bree R., Stunnenberg H.G., Chuan Peng W., Roodhart J., Minguet S., Sebestyén Z., Beringer D.X., Kuball J.
Nat Immunol. 2023 Nov 27. doi: 10.1038/s41590-023-01665-0.

A distinct topology of BTN3A IgV and B30.2 domains controlled by juxtamembrane regions favors optimal human γδ T cell phosphoantigen sensing

Karunakaran M.M., Subramanian H., Jin Y., Mohammed F., Kimmel B., Juraske C., Starick L., Nöhren A., Länder N., Willcox C.R., Singh R., Schamel W.W., Nikolaev V.O., Kunzmann V.,Wiemer A..,Willcox B.E., Herrmann T.
Nat Commun. 2023 Nov 22;14(1):7617

γδ T cells: origin and fate, subsets, diseases and immunotherapy

Hu Y., Hu Q., Li Y., Lu L., Xiang Z., Yin Z., Kabelitz D., Yangzhe W.
Signal Transduct Target Ther. 2023 Nov 22;8(1):434.

Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts

Idstein V., Ehret A.K., Yousefi O.S., Schamel W.W.
ACS Synth Biol. 2023 Oct 20;12(10):2857-2864

RORγt+ c-Maf+ Vγ4+ γδ T cells are generated in the adult thymus but do not reach the periphery

Yang T., Barros-Martins J., Wang Z., Wencker M., Zhang J., Smout J., Gambhir P., Janssen A., Schimrock A., Georgiev H., León-Lara X., Weiss S., Huehn J., Prinz I., Krueger A., Foerster R., Walzer T., Ravens S.
Cell Rep. 2023 Oct 9;42(10):113230

Transcriptomic profile of TNFhigh MAIT cells is linked to B cell response following SARS-CoV-2 vaccination

Marzano P., Balin S., Terzoli S., Della Bella S., Cazzetta V., Piazza R., Sandrock I., Ravens S., Tan L., Prinz I., Calcaterra F., Di Vito C., Cancellara A., Calvi M., Carletti A., Franzese S., Frigo A., Darwish A., Voza A., Mikulak J., Mavilio D.
Front Immunol. 2023 Jul 26:14:1208662

High-fat-diet-associated intestinal microbiota exacerbates psoriasis-like inflammation by enhancing systemic γδ T cell IL-17 production

Sonomoto K., Song R., Eriksson D., Hahn A.M., Meng X., Lyu P., Cao S., Liu N., Taudte V., Wirtz S., Tanaka Y., Winkler T.H., Schett G., Soulat D., Bozec A.
Cell Rep. 2023 Jul 25;42(7):112713

Human γδ T cell Identification from Single-cell RNA Sequencing Datasets by Modular TCR Expression

Song Z., Henze L., Casar C., Schwinge D., Schramm C., Fuss J., Tan L., Immo Prinz I.
J Leukoc Biol. 2023 Jul 12:qiad069

Breast Cancer Stem Cell-Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell Ligands

Raute K., Strietz J., Parigiani M.A., Andrieux G., Thomas O.S., Kistner K.M., Zintchenko M., Aichele P., Hofmann M., Zhou H., Weber W., Boerries M., Swamy M., Maurer J., Susana Minguet S.
Cancer Immunol Res. 2023 Jun 2;11(6):810-829

Performance comparison of TCR-pMHC prediction tools reveals a strong data dependency

Deng L., Ly C., Abdollahi S., Zhao Y., Prinz I., Bonn S.
Front Immunol. 2023 Apr 18;14:1128326

Phosphoantigen sensing combines TCR-dependent recognition of the BTN3A IgV domain and germline interaction with BTN2A1

Willcox C.R., Salim M., Begley C.R., Karunakaran M.M., Easton E.J., von Klopotek C., Berwick K.A., Herrmann T., Mohammed F., Jeeves M., Willcox B.E.
Cell Rep. 2023 Mar 28;42(4):112321

A monoclonal Trd chain supports the development of the complete set of functional γδ T cell lineages

A.M. Hahn, L. Vogg, S. Brey, A. Schneider, S.Schäfer, R. Palmisano, A. Pavlova, I. Sandrock, L. Tan, A.S. Fichtner, I. Prinz, S. Ravens, T.H. Winkler
Cell Rep. 2023 Mar 14;42(3):112253

Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions

Russ M., Ehret A.K., Hörner M., Peschkov D., Bohnert R., Idstein V., Minguet S., Weber W., Lillemeier B.F., Sascha Yousefi O.S., Schamel W.W.
Front Mol Biosci. 2023 Feb 20:10:1143274

Kidney-resident innate-like memory γδ T cells control chronic Staphylococcus aureus infection of mice

Bertram T., Reimers D., Lory N.C., Schmidt C., Schmid J., Heinig L.C., Bradtke P., Rattay G., Zielinski S., Hellmig M., Bartsch P., Rohde H., Nuñez S., Rosemblatt M.V., Bono M.R., Gagliani N., Sandrock I., Panzer U., Krebs C.F., Meyer-Schwesinger C., Prinz I., Mittrücker H-W.
Proc Natl Acad Sci USA. 2023 Jan 3;120(1):e2210490120
2022

OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells

Barros-Martins J., Bruni E., Fichtner A., Cornberg M., Prinz I.
Cytometry Part A. 2022 Oct;101(10):856-861.

Systematic pattern analyses of Vδ2+ TCRs reveal that shared "public" Vδ2+ γδ T cell clones are a consequence of rearrangement bias and a higher expansion status

Deng L., Harms A., Ravens S., Prinz I., Tan L.
Front Immunol. 2022 Sep 27:13:960920

Novel lectin-based chimeric antigen receptors target Gb3-positive tumour cells

Meléndez A.V., Velasco Cárdenas R. M-H., Strietz S.J., Siukstaite L., Thomas O.S., Tomisch J., Weber W., Kammerer B., Römer W., Minguet S.
Cell Mol Life Sci. 2022 Sep 12;79(10):513

A CMV-induced adaptive human Vδ1+ γδ T cell clone recognizes HLA-DR

Deseke M., Rampoldi F., Sandrock I., Borst E., Böning H., Ssebyatika G., Jürgens C., Plückebaum N., Beck M., Hassan A., Tan L., Demera A., Janssen A., Steinberger P., Koenecke C., Viejo-Borbolla A., Messerle M., Krey T., Prinz I.
J Exp Med. 2022 Sep 5;219(9):e20212525.

Phenotypic and functional analysis of gd T cells in the pathogenesis of human T-cell lymphotropic virus type 1 infection

Ruggieri M., Ducasa N., Juraske C., Gonzalez Polo V., Berini C., Quiroga M.F., Christopoulos P., Minguet S., Biglione M., Schamel W.W.
Front Immunol. 2022 Aug 11;13:920888.

New insights on murine γδ T cells from single-cell multi-omics

Tan L., Inácio D., Prinz I., Silva-Santos B.
Sci Bull (Beijing). 2022 Jun 15;67(11):1102-1104

δ T cells license immature B cells to produce a broad range of polyreactive antibodies

Rampoldi F., Donato E., Ullrich L., Deseke M., Janssen A., Demera A., Sandrock I., Bubke A., Juergens A., Swallow M., Sparwasser T., Falk C., Tan L., Trumpp A, Prinz I.
Cell Rep. 2022 May 24; 39(8):110854.

Integrin activation enables rapid detection of functional Vδ1+ and Vδ2+ γδ T cells

Herold N., Schöllhorn A., Feile A., Gaißler A., Mohrholz A., Pawelec G., Löffler M.W., Dimitrov S., Gouttefangeas C., Wistuba-Hamprecht K.
Eur. J. Immunol. 2022 May;52(5):730-736.

Butyrophilins: γδ T Cell Receptor Ligands, Immunomodulators and More

Herrmann T., Karunakaran M.M.
Front Immunol. 2022 Mar 17;13:876493

Stimulatory and inhibitory activity of STING ligands on tumor-reactive human gamma/delta T cells

Serrano R., Lettau M., Zarobkiewicz M., Wesch D., Peters C., Kabelitz D.
Oncoimmunology. 2022 Feb 1;11(1):2030021.
2021

αβ T cells replacing dermal and epidermal γδ T cells in Tcrd-/- mice express an MHC-independent TCR repertoire

Binz C., Bubke A., Sandrock I., Prinz I.
Eur J Immunol. 2021 Nov;51(11):2618-2632.

Immuno-antibiotics: targeting microbial metabolic pathways sensed by unconventional T cells

Ebert M., Oldfield E., Herrmann T.
Immunother Adv. 2021 Apr 5;1(1):ltab005

A fetal wave of human type 3 effector γδ cells with restricted TCR diversity persists into adulthood

Tan L., Fichtner A.S., Bruni E., Odak I., Sandrock I., Bubke A., Borchers A., Schultze-Florey C., Koenecke C., Förster R., Jarek M., von Kaisenberg C., Schulz A., Chu X., Zhang B., Li Y., Panzer U., Krebs C. F., Ravens S., Prinz I.
Sci Immunol. 2021 Apr 23;6:eabf0125.

Genetic Influence on the Peripheral Differentiation Signature of Vδ2+ γδ and CD4+ αβ T Cells in Adults

Beucke N., Wingerter S., Hähnel K., Larsen L.A., Christensen K., Pawelec G., Wistuba-Hamprecht K.
Cells. 2021 Feb 11;10(2):373.

Reply to: Vitamin C as a promoter of γδ T cells

Kouakanou L., Peters C., Kabelitz D.
Cell Mol Immunol. 2021 Feb;18(2):495.

Allogeneic Vγ9Vδ2 T-cell immunotherapy exhibits promising clinical safety and prolongs the survival of patients with late-stage lung or liver cancer

Xu Y., Xiang Z., Alnaggar M., Kouakanou L., Li J., He J., Yang J., Hu Y., Chen Y., Lin L., Hao J., Li J., Chen J., Li M., Wu Q., Peters C., Zhou Q., Li J., Liang Y., Wang X., Han B., Ma M., Kabelitz D., Xu K., Tu W., Wu Y., Yin Z.
Cell Mol Immunol. 2021 Feb;18:427-439

Microbiota-dependent expansion of testicular IL-17-producing Vγ6+ γδ T cells upon puberty promotes local tissue immune surveillance

Wilharm A., Brigas H.C., Sandrock I., Ribeiro M., Amado T., Reinhardt A., Demera A., Hoenicke L., Strowig T., Carvalho T., Prinz I., Ribot J.C.
Mucosal Immunol. 2021 Jan;14:242-252.

Direct Regulation of the T Cell Antigen Receptors’s Activity by Cholesterol

Pathan-Chhatbar S., Drechsler C., Richter K., Morath A., Wu W., OuYang B., Xu C., Schamel W.W
Front Cell Dev Biol. 2021 Jan 8;8:615996.

Direct Regulation of the T Cell Antigen Receptor's Activity by Cholesterol

Pathan-Chhatbar S., Drechsler C., Richter K., Morath A., Wu W., OuYang B., Xu C., Schamel W.W.
Front Cell Dev Biol. 2021 Jan 8;8:615996.
2020

A glance over the fence: Using phylogeny and species comparison for a better understanding of antigen recognition by human γδ T-cells

Herrmann T., Karunakaran M.M., Fichtner A.S.
Immunol Rev. 2020 Nov;298(1):218-236.

From basic research to clinical application of γδ T cells

Déchanet-Merville J., Prinz I.
Immunol Rev. 2020 Nov;298(1):5-9.

Tumor resistance mechanisms and their consequences on γδ T cell activation

Wesch D., Kabelitz D., Oberg H.H.
Immunol Rev. 2020 Nov;298:84-98.

Ligand recognition by the γδ TCR and discrimination between homeostasis and stress conditions

Deseke M., Prinz I.
Cell Mol Immunol. 2020 Sep;17(9):914-924.

Cancer immunotherapy with γδ T cells: many paths ahead of us

Kabelitz D., Serrano R., Kouakanou L., Peters C., Kalyan S.
Cell Mol Immunol. 2020 Sept;17:925-939.

Microbial exposure drives polyclonal expansion of innate γδ T cells immediately after birth

Ravens S., Fichtner A.S., Willers M., Torkornoo D., Pirr S., Schöning J., Deseke M., Sandrock I., Bubke A., Wilharm A., Dodoo D., Egyir B., Flanagan K.L., Steinbrück L., Dickinson P., Ghazal P., Adu B., Viemann D., Prinz I.
Proc Natl Acad Sci U S A. 2020 Aug 4;117:18649-18660.

Galectin-3 Released by Pancreatic Ductal Adenocarcinoma Suppresses γδ T Cell Proliferation but Not Their Cytotoxicity

Gonnermann D., Oberg H.H., Lettau M., Peipp M., Bauerschlag D., Sebens S., Kabelitz D., Wesch D.
Front Immunol. 2020 Jun 30;11:1328.

An Update on the Molecular Basis of Phosphoantigen Recognition by Vγ9Vδ2 T Cells

Herrmann T., Fichtner A.S., Karunakaran M.M
Cells. 2020 Jun 9;9(6):1433.

Immune Signatures and Survival of Patients With Metastatic Melanoma, Renal Cancer, and Breast Cancer

Wistuba-Hamprecht K., Gouttefangeas C., Weide B., Pawelec G.
Front Immunol. 2020 June 9;11:1152.

A comparative view on vitamin C effects on αβ- versus γδ T-cell activation and differentiation

Peters C., Kouakanou L., Kabelitz D.
J Leukoc Biol. 2020 Jun;107:1009-1022.

TCR repertoire analysis reveals phosphoantigen-induced polyclonal proliferation of Vγ9Vδ2 T cells in neonates and adults

Fichtner A.S., Bubke A., Rampoldi F., Wilharm A., Tan L., Steinbrück L., Schultze-Florey C., von Kaisenberg C., Prinz I., Herrmann T., Ravens S.
J Leukoc Biol. 2020 Jun;107(6):1023-1032

αβ and γδ T cell receptors: Similar but different.

Morath A., Schamel W.W.
J Leukoc Biol. 2020 Jun;107:1045-1055.

Pitfalls in the characterization of circulating and tissue-resident human γδ T cells

Beucke N., Wesch D., Oberg H.H., Peters C., Bochem J., Weide B., Garbe C., Pawelec G., Sebens S., Röcken C., Hashimoto H., Löffler M.W., Nocerino P., Kordasti S., Kabelitz D., Schilbach K., Wistuba-Hamprecht K.
J Leukoc Biol. 2020 Jun;107:1097-1105.

Bispecific antibodies enhance tumor-infiltrating T cell cytotoxicity against autologous HER-2-expressing high-grade ovarian tumors

Oberg H.H., Janitschke L., Sulaj V., Weimer J., Gonnermann D., Hedemann N., Arnold N., Kabelitz D., Peipp M., Bauerschlag D., Wesch D.
J Leukoc Biol. 2020 Jun;107:1081-1095.

Resolving the mystery-How TCR transgenic mouse models shed light on the elusive case of gamma delta T cells

Hahn A.M., Winkler T.H.
J Leukoc Biol. 2020 Jun;107:993-1007.

Influence of Indoleamine-2,3-Dioxygenase and Its Metabolite Kynurenine on γδ T Cell Cytotoxicity against Ductal Pancreatic Adenocarcinoma Cells

Jonescheit H., Oberg H.H., Gonnermann D., Hermes M., Sulaj V., Peters C., Kabelitz D., Wesch D.
Cells. 2020 May 6;9:1140.

Accurate determination of γδ T cells in multi-channel mass and flow cytometry

Beucke N., Wistuba-Hamprecht K.
Cytometry B Clin Cytom. 2021 May;100(3):288-289.

Vitamin C promotes the proliferation and effector functions of human γδ T cells

Kouakanou L., Xu Y., Peters C., He J., Wu Y., Yin Z., Kabelitz D.
Cell Mol Immunol. 2020 May;17:462-473.

Vitamin C supports conversion of human γδ T cells into FOXP3-expressing regulatory cells by epigenetic regulation

Kouakanou L., Peters C., Sun Q., Floess S., Bhat J., Huehn J., Kabelitz D.
Sci Rep. 2020 Apr 16;10:6550.

Butyrophilin-2A1 Directly Binds Germline-Encoded Regions of the Vγ9Vδ2 TCR and Is Essential for Phosphoantigen Sensing

Karunakaran M.M., Willcox C.R., Salim M., Paletta D., Fichtner A.S., Noll A., Starick L., Nöhren A., Begley C.R., Berwick K.A., Chaleil R.A.G., Pitard V., Déchanet-Merville J., Bates P.A., Kimmel B., Knowles T.J., Kunzmann V., Walter L., Jeeves M., Mohammed F., Willcox B..E., Herrmann T.
Immunity. 2020 Mar 17;52(3):487-498.e6

Human γδ TCR Repertoires in Health and Disease

Fichtner A.S., Ravens S., Prinz I.
Cells. 2020 Mar 26;9(4):800.

Alpaca (Vicugna pacos), the first nonprimate species with a phosphoantigen-reactive Vγ9Vδ2 T cell subset

Fichtner A.S., Karunakaran M.M., Gu S., Boughter C.T., Borowska M.T., Starick L., Nöhren A., Göbel T.W., Adams E.J., Herrmann T.
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6697-6707.

Translating gammadelta (γδ) T cells and their receptors into cancer cell therapies

Sebestyen Z., Prinz I., Déchanet-Merville J., Silva-Santos B., Kuball J.
Nat Rev Drug Discov. 2020 Mar;19:169-184.
2019

The TCR is an allosterically regulated macromolecular machinery changing its conformation while working

Schamel W.W., Alarcon B., Minguet S.
Immunol Rev. 2019 Sep;291:8-25.

TRAIL-Receptor 4 Modulates γδ T Cell-Cytotoxicity Toward Cancer Cells

Tawfik D., Groth C., Gundlach J.P., Peipp M., Kabelitz D., Becker T., Oberg H.H., Trauzold A. Wesch D.
Front Immunol. 2019 Aug 28;10:2044.

Dangerous γδ T cells in aged mice

Prinz I., Sandrock I.
EMBO Rep. 2019 Aug;20:e48678

High-throughput analysis of the human thymic Vδ1+ T cell receptor repertoire

Di Lorenzo B., Ravens S., Silva-Santos B.
Sci Data. 2019 Jul 4;6:115.

Single-Cell Transcriptomics Identifies the Adaptation of Scart1+ Vγ6+ T Cells to Skin Residency as Activated Effector Cells

Tan L., Sandrock I., Odak I., Aizenbud Y., Wilharm A., Barros-Martins J., Tabib Y., Borchers A., Amado T., Gangoda L., Herold M.J., Schmidt-Supprian M., Kisielow J., Silva-Santos B., Koenecke C., Hovav A.H., Krebs C., Prinz I., Ravens S.
Cell Rep. 2019 Jun 18;27:3657-3671.e4.

Styk1 is specifically expressed in NK1.1+ lymphocytes including NK, γδ T, and iNKT cells in mice, but is dispensable for their ontogeny and function

Wilharm A., Sandrock I., Marotel M., Demera A., Naumann R., Walzer T., Prinz I.
Eur J Immunol. 2019;49:686-693.